What is vector transfection?

Transfection is most commonly performed to express a protein of interest in cultured cells (or an animal model) through the use of a plasmid vector or mRNA.

What is transfection of mammalian cells?

Mammalian cell transfection is a technique commonly used to express exogenous DNA or RNA in a host cell line (for example, for generating RNAi probes). There are many different ways to transfect mammalian cells, depending on the cell line characteristics, desired effect, and downstream applications.

Can plasmid be transfection into mammalian cells?

Plasmid transfection into mammalian cells is fairly straightforward and the resultant cells can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material directly into the genome to form a stable transfection.

What is the term for the uptake and expression of foreign DNA using mammalian cells?

Transfection is the process by which foreign DNA is deliberately introduced into a eukaryotic cell through non-viral methods including both chemical and physical methods in the lab.

Do mammalian cells have plasmids?

Plasmids are small circular DNA molecules that naturally occur in bacteria, and are actually used by the bacteria to transfer genetic information. The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection.

What is a mammalian cell?

Mammalian cells are cells that are derived or isolated from tissue of a mammal. In this experiment, students are introduced to four mammalian cell types: fibroblasts, epithelial cells, lymphocytes and macrophages. Lymphocytes are found within the blood.

What is transfection biotechnology?

Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.

What is vector transfection in microbiology?

Vector (molecular biology) Insertion of a vector into the target cell is usually called transformation for bacterial cells, transfection for eukaryotic cells, although insertion of a viral vector is often called transduction.

What are viral vectors?

Viral vector. Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism ( in vivo) or in cell culture ( in vitro ). Viruses have evolved specialized molecular mechanisms to efficiently transport their genomes inside the cells they infect.

What is an in vivo vector?

Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism ( in vivo) or in cell culture ( in vitro ). Viruses have evolved specialized molecular mechanisms to efficiently transport their genomes inside the cells they infect.

What is a vector in molecular biology?

Vector (molecular biology) In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g.- plasmid, cosmid, Lambda phages ). A vector containing foreign DNA is termed recombinant DNA.